In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes
نویسندگان
چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Cryobiological effects of cryoprotectants on morphology of cumulus oocyte complexes (COCs) of sheep using vitrification
The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...
متن کاملCryobiological effects of cryoprotectants on morphology of cumulus oocyte complexes (COCs) of sheep oocytes using vitrification
The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocytes using vitrification. The vitrification of sheep oocytes was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more...
متن کاملIn vitro effects of relaxin on gene expression in porcine cumulus-oocyte complexes and developing embryos
BACKGROUND Relaxin hormone peptide is found in porcine follicular and utero-tubal fluids, but its possible actions during early embryo development are still undetermined. Here, we investigated the effects of porcine relaxin during oocyte maturation and embryo development, and gene expression in the pig. METHODS Immature cumulus-oocyte complexes (COCs) were obtained from ovarian follicles of s...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2010
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-010-9401-z